本文主要展示如何利用R语言绘制来自nature期刊ABO genotype alters the gut microbiota by regulating GalNAc levels in pigs(Hui Yang, et al,2022)[1]一文中的Extended Data Fig.2a。该图形式为条形图,用于展示微生物群数量。如下图所示:

1、图形绘制
library(dplyr)
library(ggplot2)
ED.fig2 <- read.table("core_OTUs_annotation_result.txt",header = TRUE )
ED.fig2$level <- factor(ED.fig2$level, levels = c("order", "family", "genus" ))
ED.fig2 <- ED.fig2 %>% group_by(level) %>% arrange(level, -number) %>% ungroup() as.data.frame() #分组降序
ED.fig2$taxa <- factor(ED.fig2$taxa, levels = ED.fig2$taxa)
ED.fig2
# taxa number level
#1 Bacteroidales 6 order
#2 Clostridiales 3 order
#3 Ruminococcaceae 7 family
#4 Lachnospiraceae 6 family
#5 RF16 2 family
#6 Clostridiaceae 1 family
#7 Mogibacteriaceae 1 family
#8 S24-7 1 family
#9 Parabacteroides 7 genus
#10 Oscillospira 6 genus
#11 Prevotella 5 genus
#12 Sphaerochaeta 4 genus
#13 Bacteroides 1 genus
#14 CF231 1 genus
#15 p-75-a5 1 genus
#16 Phascolarctobacterium 1 genus
#17 Roseburia 1 genus
#18 SMB53 1 genus
#19 Treponema 1 genus
#20 Turicibacter 1 genus
#21 YRC22 1 genus
# 绘图
ggplot(ED.fig2, aes(number, taxa, fill = level)) +
geom_col() +
geom_text(aes(label = number), hjust = -0.5, size = 4.5) +
theme_bw() +
scale_fill_manual(values = c("#F7931E","#0170BB", "#019244")) +
scale_x_continuous(expand = c(0.02, 0), limits = c(0,7.5)) +
theme(panel.grid = element_blank(),
legend.position = c(0.99, 0.99),
plot.title = element_text(size = 18, hjust = 0.5),
legend.justification = c(1,1),
legend.text = element_text(size = 12),
legend.title = element_text(size = 15),
axis.ticks = element_line(size = 2),
#axis.ticks.length = unit(0.1,"cm"),
axis.title.y = element_blank(),
axis.title.x = element_text(size = 15),
axis.text.y = element_text(angle = -30, vjust = 1),
axis.text = element_text(size = 12, colour = "black")) +
labs(x = "Number of share core otu", title = "OTU Annotation")

该图的一个关键点在于,虽然该图按number顺序进行升序排序,不过是在不同因子内部进行升序排序。此处用到了dplyr包中的函数对数据进行了预处理,对数据进行了分组排序,从而实现了图中所呈现的数据顺序。关于分组排序具体可进一步阅读推文R数据分析|分组排序。
2、其他
其他绘图方法可进一步阅读公众号其他文章。
如有帮助请多多点赞哦!
参考资料
Yang, H., Wu, J., Huang, X. et al. ABO genotype alters the gut microbiota by regulating GalNAc levels in pigs. Nature 606, 358–367 (2022). : https://doi.org/10.1038/s41586-022-04769-z
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